Application

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp ^™ extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp^™ PCR Reaction Mix.

General description

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp^™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Packaging

24 mL in poly bottle